P#23   TGF-b 1 and IGF-1 regulated gene expression profile analysis in human outer root sheath cells

Young-Hee Kim, Moonkyu Kim, So-Young Cha, Sang-Uk Im, Jung-Chul Kim. Hair Research Center, Kyungpook National University - School of Medicine, Korea

Hair follicle is a highly sensitive mini-organ whose cyclic transformations from phases of anagen, catagen and telogen are profoundly influenced by numerous factors such as growth factors, cytokines, hormones, neuropeptides, and pharmaceutical products. TGF-b 1 and IGF-1 have been suggested to play a role in regulating the development and cycling of hair follicle. And outer root sheath (ORS) cells have been shown to generate and receive many signals, thus plays an important role in hair cycle. In this work, to identify changes in gene expression caused by IGF-1 or TGF-b 1 in beard or scalp ORS cells, we used cDNA microarray analysis. Microarray containing 3,063 human cDNA clones was hybridized with fluorescently labeled cDNA probes. Twenty five clones were up-regulated and 36 genes were down-regulated in TGF-b 1 stimulated ORS cells. Nine genes were up-regulated and 27 genes were down-regulated in IGF-1 stimulated ORS cells. The genes that were identified as up-regulated by TGF-b 1 are BIGH3, TCF3, nexin, collagen type I, versican, osteoblast specific factor 2, 78 kDa gastrin-binding protein, N-cadherin, NNP-1. Colligin2, TSC-22 related protein, and snRNP E are up-regulated by IGF-1. IGFBP3, tyrosinase-related protein 1, IL-1b, TNF-a, calpain, calmodulin, IL-4 receptor, and fibronectin receptor genes are down-regulated by TGF-b 1, and keratin 23, chromatin assembly factor-1 p60 subunit, ralA-binding protein, short type of multidrug resistance protein homolog, ERCC2 genes were down-regulated by IGF-1. Further analysis should certainly provide new connections between growth factors and cellular pathways that cannot be anticipated by our current limited knowledge of the genes.