P#30  Examination of urinary steroid metabolites in men with male-pattern alopecia

Eniko Telegdy1, Viktoria Poór2, Susanne Juricskay2 and Beatrix Farkas1. 1Dept of Dermatology, Faculty of Medicine,University Pècs, Hungary. 2Central Research Laboratory, University Pècs, Hungary

23 men were examined (aged 25.1 ± 7.7 years ) with androgenic alopecia. Enzyme hydrolysis, solid phase extraction, methoxym-silyl derivatization and capillary gas chromatographic analysis were used to examine the changes in urinary steroid metabolites. Significantly increased values were found in the metabolites of testosterone, androsterone (p<0.02) and etiocholanolone (p<0.05) in patients with androgenic alopecia, compared to the control values. Elevated levels of 16-hydroxy-dehydroepiandrosterone (16-OHD)(p<0.03) and cortisol (p<0.05) were found, but the levels of cortisol metabolites were unchanged. Calculating the ratio of total 5a / 5b metabolites provided information on the activity of 5a-reductase activity. The elevated 16-OHD level could be indicative of patients who had mild hyperadrenal activity.