P#33  Changes of several immunological markers in human hair cycle

Jun-Kyu Oh, Mi-Hyang Kim, Chang-Hun Huh, Kyu-Han Kim, Hee-Chul Eun. Dept of Dermatology, Seoul National University, College of Medicine, Seoul, Korea

The studies about morphological and molecular changes occur during hair cycle would be a good basic experimental model about organogenesis and apoptosis as well as development of new drugs which could modify hair growth activity. Most reports about hair cycle have been done with murine models and little is known about human hair cycle because getting enough numbers of human catagen and telogen hair follicles is very difficult. We have investigated changes of some immunological markers using a method to select catagen and telogen hair follicles from adult human scalp tissues. We have made our own selection criteria for catagen and telogen hair follicles. Based on these criteria, we were able to group dissected living hair follicles into anagen, early catagen, mid-catagen, late catagen, and telogen. Grouped follicles were embedded in Tissue-Tek OCT compound, snap-frozen over liquid nitrogen, and stored at -70°C until used for histological studies about expression of MHC I, MHC II, CD 1a, ACTH, TGF-b1,2,3. Using these method and criteria, we think we can get enough numbers of substaged catagen and telogen hair follicles from a number of different donors for another large-scale study about human hair cycle.