P-04   EFFECT OF CALCITONIN GENE RELATED PEPTIDE ON THE HUMAN HAIR GROWTH IN VITRO

W-S Lee, E. Lee, S. Lee Department of Dermatology and Institute of Hair and Cosmetic Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.

Numerous growth factors and their interaction with receptors affect the hair growth and hair cycle. Recently, it has been reported that pheripheral nervous system has important roles in the hair growth and hair cycle change, which are mediated by neuropeptides. Calcitonin gene related peptide(CGRP) is one of the most important neuropeptides, but the effect of CGRP on hair growth has rarely reported. In this study, we examined the effect of CGRP on human hair growth using organ culture system. We also examined molecular mechanism of the effect of CGRP in hair follicles and hair dermal papilla cells. In order to identify the molecular mechanism, we detected mRNAs corresponding to FGF-7, IGF-1, VEGF, IL-1alpha, IL-1beta, p53, caspase-3. RT-PCR analysis showed that CGRP induced expressions of mRNA levels of IL-1alpha in human hair follicles and dermal papilla cells. In addition, the CGRP inhibited expression of FGF-7 in human hair follicles.