P2.20 RECONSTRUCTION OF A SHEET CONSISTING OF DERMAL PAPILLA CELLS IN VITRO

Department of Dermatology, Sungkyunkwan University School of Medicine, Samsung Medical Center, Seoul, Korea

Dermal papilla cells play an important role in the development and growth of hair follicles. In the past, monolayer cultures have been used for the study of biological properties of dermal papilla cells. However, conventional submerged monolayer cultures have several limitations. In this study, to reconstruct a three-dimensional morphology consisting of dermal papilla cells in vitro, dermal papilla cells were cultured in the medium containing several supplements. Several week later, histology and immunohistochemistry were performed.

Cultured dermal papilla cells produced a sheet consisting of dermal papilla cells macroscopically. Histologically, many dermal papilla cells were found between extracellular matrixes in the sheet. Immunohistochemically, extracellular matrix such as procollagen 1 and tropoelastin were expressed. Vimentin and smooth muscle actin were also expressed, similar to dermal papilla cells in vivo.

These findings show that the sheet consisting of dermal papilla cells mimics dermal papilla in vivo, suggesting that the sheet may be useful for the study of biological properties of dermal papilla cells.