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P4.147 Detection of EV-HPV DNA in Hairfollicles of different Localizations in Transplanted patients vs. controls Schmook T.1, Nindl I.1, Köhler A.1, Hezel S.1, Meyer T.2, Sterry, W. 1, Stockfleth E.11Department of Dermatology, Charité, University Hospital, D-10117 Berlin, Germany 2IPM-HH, D-22339 Hamburg, Germany Objective: It is established that Human Papillomaviruses (HPV) are an important cofactor in development of anogenital tumors and for some skin tumours, like in epidermodysplasia verruciformis (EV) and skin tumours in organ transplant patients (TX). Since skin tumours in TX are an increasing life-threatening problem, the reservoir and role of HPV is of accretive interest. Hairfollicles of the eyebrow are the reservoir of certain HPV types. But it is still unclear whether other localizations of hairfollicles could be also serve as a reservoir of different oncogenic HPV-types. Patients and Methods: We examined the presence of 24 different oncogenic cutaneous HPV types in hair of 5 different regions on the body. Sun-exposed areas like eyebrow and head, low sun-exposed areas (abdomen, lower leg), and sun-protected pubic area. Five hairs of each localization were collected under sterile conditions. 9 kidney transplanted patients vs. 9 non-immunosuppressed patients without HPV associated skin diseases were examined. Patients were matched by age (44 to 75 ys.) and gender (5 women, 13 men). HPV analysis was performed by using a L1 PCR-based method to detect HPV types (B1 and B2 group) followed by typing reverse line blotting, only ß-globin (PCR) positive samples were included.Results: HPV DNA was found in every sample of TX whereas in the control group only 4 (out of 9) patients showed positive results. HPV detection in TX were much higher in relative quantity and also in diversity of HPV types. TX showed mainly HPV types 5, 8, 15, 23, 37; the control group mainly 14 and 19. In TX were no evident difference of HPV-type pattern in relation to region and sun-exposition. The control had a higher amount of different HPV-types in eyebrows and head than in lower regions. Conclusion: Since oncogenic cutaneous HPV-types might play a role as cofactor in skin carcinogenesis in organ transplant recipients, our results point out that different locations of hairfollicles can serve as a HPV reservoir. Skin tumours in TX-patients are located on sun exposed skin areas, therefore it seems that multiple factors like immunosuppression, UV-light and HPV work together as cofactors in skin carcinogenesis. |
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