P34 ANALYSIS OF HAIR SOLUBLE PROTEIN FROM EXTRINSIC HAIR DAMAGE
Jeon SY, Lee WS
Department of Dermatology and Institute of Hair and Cosmetic Medicine, Yonsei University Wonju College of Medicine, Korea

The cosmetic procedures such as permanent waving or hair dyeing are popular today, but the medical studies related to the meaning of the hair damage and the restoration pattern from damage are mainly based on the structural findings. The purpose of this study was to identify the patterns of hair damage and restoration through electrophoresis and western blot analysis of the hair proteins. We conducted permanent waving on three volunteers, hair dyeing on two volunteers, and both permanent waving and hair dyeing on two volunteers. We performed extraction and concentration of the whole and partial hair protein, and then operated electrophoresis and western blot analysis of the hair protein. The following results were obtained from this study: (1) In the western blot analysis of whole hair proteins, there are no positive findings except one person who permed his hair. It may result from the small amount of partial hair proteins among the whole hair proteins. (2) In the western blot analysis of partial hair proteins, there are five positive findings on the day immediately after hair damage. All of them showed one positive finding at least during the experiment. On the other hand, 4 subjects showed positive findings on the 12th and 14th week after the treatment which did not show during the middle course of the treatments. We think these findings present continuous hair damage in the moleculobiologic aspect until 14th week. These results showed change of the hair proteins from hair damage, and we found the possibility of prolongation of actual hair damage longer than expected ultrastructurally.