13.    Induction of synapse associated protein 102 (SAP102) expression in cyclosporin A-stimulated hair growth. 
Jang-Kyu Park, Chang Deok Kim, Myung Im, Young-Joon Seo, Young-Joon Seo,   Department of Dermatology, Chungnam National University, Daejeon, Korea

Cyclosporin A (CsA) has been used as a potent immunosuppressive agent for inhibiting the graft rejection after organ transplantation. However, CsA provokes lots of side effects including hirsutism, the phenomenon of abnormal hair growth in the body. In this study, we attempted to identify the genes related with CsA-induced hair growth. When topically applied on the back skin of C57BL/6 mice, CsA produced prominent hair growth stimulating effect. Using differential display reverse transcription-polymerase chain reaction (DD RT-PCR) technique, several genes were identified to be differentially expressed by CsA. Among those, the expression of SAP102 was verified using competitive RT-PCR. The result showed that the expression of SAP102 was significantly induced by CsA treatment in the back skin of C57BL/6 mice. However, the increase of SAP102 mRNA was also seen in spontaneous anagen mice, suggesting that induction of SAP102 is one event of the anagen hair growth response regardless of how the growth state was induced. SAP102 was not expressed in cultured human hair outer root sheath and dermal papilla cells. Immunohistochemistry analysis showed that CsA induced the expression of SAP102 in perifollicular region of mouse anagen hair. These results suggest that SAP102 is induced in anagen phase of hair cycle and have an indirect role in the hair growth.